Syed Raziuddin Quadri1,2,3, Jamal Al Ruwaili1,Shaik Mohammed Akbar5, Wen-Jun Li3,4 and Dayanand Agsar2 Sixty three actinobacteria were isolated from Limestone quarries of Deccan Traps from South India. The isolates were identified by morphological, biochemical and physiological studies. Among these, six isolates were selected on the basis of their amylolytic activity using submerged cultivation. The potential isolates exhibiting amylolytic activity were DRQ10, DRQ20, DRQ86, DRQ33, DRQ41, and DRQ63 as confirmed by formation of clear zones of starch hydrolysis around the colonies, with maximum activities 2500, 1100, 1200, 1150, 1250, 1400, and 1600 Units, respectively, as determined by DNS (3...

Deene Manik Prabhu1,2,7, Syed Raziuddin Quadri3,7, Juan Cheng4, Lan Liu4, Wei Chen5, Ying Yang5,Wael N Hozzein6, Kattimani Lingappa2 and Wen-Jun Li1,4 A moderate growing strain designated as MPKL 26 T was isolated from a soil sample of Bidar Fort, Karnataka, India. The strain MPKL 26T was Gram positive, bent rod in shape. The optimum pH and temperature for growth was 7.0 and 30 °C, respectively. The 16S ribosomal RNA gene sequence analysis revealed that strain MPKL 26 T was closely related to S. atrocyanea DSM 20127T (98.09%), S. flava CW 108T (98.04%), S. soli CW 59T (97.99%) and S. notoginsengisoli SYP-B575T (97.0%) and showed DNA–DNA hybridization relatedness (46.05 ±1.2, 33.56 ±2.55, 32...

S. G. Dastager Æ Agasar Dayanand Æ Wen-Jun Li Æ Chang-Jin Kim Æ Jae-Chan Lee Æ Dong-Jin Park Æ Xin-Peng Tian Æ Q. S. Raziuddin Received: 9 April 2007 / Accepted: 13 January 2008
Springer Science+Business Media, LLC 2008 Abstract Multiple proteases were produced and partially purified from an alkali-thermotolerant novel species of Streptomyces (i.e., Streptomyces gulbargensis DAS 131) after 48 h of growth at 45C. The enzyme preparation exhibited activity over a broad range of pH (4–12) and temperature (27–55C). Optimum activity was observed at a pH of 9.0 and a temperature of 45C. Starch and protease peptone was found to be a good source of carbon and nitrogen to enhance the enzyme...